经过评估，专家小组认为该添加剂对目标物种、消费者和环境是安全的。由于缺乏数据，研究小组无法就该添加剂对皮肤或眼睛产生刺激的可能性得出结论。评估中的添加剂被认为是非反刍动物物种氨基酸L-异亮氨酸的有效来源。为了使补充的L-异亮氨酸对反刍动物和非反刍动物同样有效，需要防止其在瘤胃中降解。部分原文报道如下：

 

　　Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of L-isoleucine produced with Escherichia coli CCTCC M 20231916 as a nutritional feed additive for all animal species. The production strain E. coli CCTCC M 20231916 is genetically modified. No viable cells nor DNA of the production strain are detected in the final product. Therefore, the additive does not pose any safety concern regarding the production strain. The use of L-isoleucine produced by fermentation with E. coli CCTCC M 20231916 in feed is safe for the target species when supplemented in appropriate amounts to the diet according to their nutritional needs. The FEEDAP Panel has concerns about the use of L-isoleucine in water for drinking. The use of L-isoleucine produced by fermentation with E. coli CCTCC M 20231916 in animal nutrition is considered safe for consumers and the environment. As regards the safety for the user, the FEEDAP Panel cannot conclude on the potential of the additive to be irritant to skin and/or eyes and to be a potential skin sensitiser. The feed additive consisting of L-isoleucine produced by fermentation with E. coli CCTCC M 20231916 is regarded as an effective source of the amino acid L-isoleucine for all non-ruminant species. For the supplemental L-isoleucine to be as efficacious in ruminants as in non-ruminant species, it requires protection against degradation in the rumen.

 

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